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EZ-96 DNA Methylation-Direct Kit, Deep-Well, 2 x 96 Reactions

Home Zymo Epigenetics EZ-96 DNA Methylation-Direct Kit, Deep-Well, 2 x 96 Rxns
Price$ 595.48

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Deep-Well $ 595.48 Shallow-Well $ 595.48
  • SKU: ZD5023
  • Pack Size: Deep-Well
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EZ-96 DNA Methylation-Direct Kit, Deep-Well, 2 x 96 Reactions
Description Specifications

Room Temperature


The EZ-96 DNA Methylation-Direct Kit is a further refinement of our popular EZ DNA Methylation and EZ DNA Methylation-Gold kits. These products feature reliable and complete DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity of these kits make it possible to amplify bisulfite-converted DNA from as few as 10 cells or 50 pg DNA. Like the EZ DNA Methylation-Gold kits, DNA denaturation and bisulfite conversion processes are combined into a single step. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including restriction endonuclease digestion, sequencing, microarrays, etc.

High-throughput 96-well bisulfite conversion of DNA directly from blood, soft tissue, cells, FFPE samples, and LCM samples.
Compatible with small sample inputs as few as 10 cells or 50 pg DNA.
Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.

5 lbs

16.00 x 14.00 x 8.00



Applications: Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc.
Conversion: > 99.5%
Elution Volume:
 Shallow well: > 30 µl
 Deep-well: >15 µl
Equipment: Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers.
Input: Samples containing between 50 pg to 2 µg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng. The number of cells per standard treatment can range from 10-105 cells.
Processing Time: 4 hours
Recovery: > 80%
Sample Source: Blood, tissue, cells, FFPE, LCM-derived samples, purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc.

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