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EZ-96 DNA Methylation Kit, Deep Well Format, 2 x 96 Reactions

Price$ 511.95
  • SKU: ZD5004
  • Pack Size: Deep Well
Quantity
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EZ-96 DNA Methylation Kit, Deep Well Format, 2 x 96 Reactions

Room Temperature


The EZ-96 DNA Methylation Kits are bisulfite conversion kits that feature a high-throughput (96-well spin-plate), simplified procedure that streamlines bisulfite conversion of DNA. Cytosines undergo a three-step reaction with sodium bisulfite during which the cytosine is converted into uracil. The innovative in-column desulphonation technology eliminates otherwise cumbersome precipitations. The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for downstream analyses including PCR amplification, endonuclease digestion, sequencing, microarrays, etc. Note: Catalog # ZD5004 is recommended for use with the Illumina Infinium MethylationEPIC BeadChip array.

Streamlined, proven procedure for bisulfite conversion of DNA.
Desulphonation and recovery of bisulfite-treated DNA with a 96-well spin-column plate.
Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.

3 lbs

9.00 x 7.00 x 7.00



Applications: Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc. Catalog # ZD5004 is recommended for use with Illumina Infinium MethylationEPIC BeadChip array.
Conversion: >99%
Elution Volume: ≥ 15 µl for Deep-well ≥ 30 µl for Shallow-well
Equipment: Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers.
Input: 500 pg - 2 µg of DNA
Processing Time: 12-16 hours
Recovery: >80%
Sample Source: Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.

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